Approximate total - 650!

9/27
20 BMP 2.2 1:5 dilution from 9/22
~8 SFD RNA 4.5 div (0.1%) of 1:1 dilution
7 div calcein into ~8 unfertilized in choline SW + 20 mM CoCl
7 div beta catenin siRNA into ~20 unfertilized in choline SW + CoCl

9/26
20 BMP 2.2 1:5 dilution from 9/22

9/25
20 BMP 2.2 1:5 dilution from 9/22
10 otx-1 (chemical siRNA)

9/24
10 otx dicer (by mistake)
10 otx-2 (chemical siRNA)
10 wnt1-1
10 wnt1-2
6 sfd 1%
6 sfd 0.5%
> 30 six-3 1%
11 six-3 0.25%
10 six-3 0.06%

9/23
can't get fertilization!!

9/21
FGF8-2 synthetic siRNA 1:10 dilution
9/20
3 x 4 beta catenin dilutions from 1:10 (1%, 0.25%, 0.06%)
35 BMP 2.2

9/19 finish around 11:40
uninjected
1% (14 div)
0.5% (10 div)
0.25% (7 div)
0.125% (5 div)
0.06% (3.5 div)
10 FGFR "sfd" RNA
10 FGF8-1 chem siRNA 1:10

9/18
10 sfrp RNA
10 six-3 #1
24 BMP 2.2 1:10 dilution

9/17
10 Xwnt?
10 X axin
10 X beta catenin

9/16
10 dkk chem si #1
12 beta catenin ds RNA
13 DMBX
10 FGF8L chem si RNA
10 wnt8-2 chem si RNA
10 wnt8-1 chem si RNA

9/15
10 noggin + chordin
10 chem si RNA six-3 #1
10 chem si RNA six-3 #1
9 FGFR RNA 1:10 dilution

9/14
30 beta catenin
20 sfrp RNA

9/13
10 chordin
10 brachyury
10 wnt 1 RNA
10 six 3 RNA
10 six 3
4 wnt8 rna (0.5%)
4 wnt8 rna (0.25%)

9/12
10 beta catenin
10 FGF 8-5
9 Wnt 8 RNA
10 sfrp RNA
10 dkk
11 pitx2
10 dmbx