Saccoglossus injection and time lapse
Eggs were injected in the first 6 min after fertilization by a front loading method.
Time lapse and other images, movies
Fertilization - compound scope, first minute. Images at 1 sec intervals
Calcium increase at fertilization Unfortunately,
the stage was moved just before the first calcium increase. It seems that
there is a small increase followed by a large increase. It doesn't look like
there is a calcium wave. (n = 1, so take with a grain of salt)
Cleavages, from 7 min after fertilization to 10th/11th
cleavage. Original movie was shot at 600 pixels x 600 pixels, it was reduced
to 300 x 300 in order to make the movie size smaller.
Pictures of the worm
outside of the sand, showing the orange/pink proboscis, red orange collar and
hind part
other pictures 1 -- 2 -- 3 -- 4
odds and ends
Fertilization cone
polarity staining by TMRE? Staining was started at 1 hr after fertilization
(200 nM leave in sea water), observed at 1 hr 40 min (TMRE = tetramethy rhodamine
ester, has been used for staining mitochondria in other cells)
low mag polarity 1 -- 2
higher mag polarity showing correspondence of fluorescence and extracellular
coat
surface staining at high mag, perhaps showing boundary
loss of staining, at 1 hr 50 min
Saccoglossus is a dim egg: these are graphs of the
brightness in a z series of a starfish oocyte (upper) and saccoglossus (lower)
embryo, both injected with calcein. The fluorescence can
be detected much deeper in a starfish oocyte
