Microscopy Resources

Microscope Companies
Leica
Nikon
Olympus
Zeiss

Laser scanning confocal microscopes
Leica (4 pi, TCS SP5)
Nikon C1 plus confocal microscope
Nikon LiveScan SFC
Olympus (Fluoview 1000 dual scan, Fluoview 300, Spinning disk)
Zeiss (5 Live, 510, Meta, Confocor 3)

Olympus spinning disk
Solamere
Perkin Elmer Ultraview Spinning disk microscope

Web microscopy sites
Nikon Microscopy U
Olympus Microscopy Resource Center
Molecular Expressions: exploring the world of optics and microscopy
Zeiss - Microscopy from the very beginning

Optical sectioning microscopy - Conchello and Lichtman
Fiber optic fluorescence imaging - Flusberg et al
Deep tissue two photon microscopy - Helmchen and Denk
Fluorescence microscopy - Lichtman and Conchello
A guide to selecting fluorescent proteins - Shaner, Steinbach and Tsien

Article from R. Tsien on localization of proteins Science 312: 5771 (2006)
Douglas Murphy book "Fundamentals of light microscopy and electronic imaging" Amazon $80.91
Other books

Cornell Multiphoton Excitation Site (with multiphoton cross section data, etc)
CARS microscopy (Sunny Xie)
STED (Stephan Hell)
Patterned illumination (Mats G. Gustafsson)

Optical filters
Chroma Technology -- Paul Milman (pmilman@chroma.com); Michael Stanley (m@chroma.com)
-- Handbook of optical filters for fluorescence microscopy
-- Handbook of filter sets for fluorescent proteins
-- Application notes on FRET
Omega Optical
Semrock

Mercury arc light source for fluorescence microscopes
EXFO

Ti Sapphire companies
Coherent
Spectra-Physics
Kapteyn-Murnane

Optical components
Newport
Thor Labs
New Focus
Melles Griot
Edmund

Siskiyou

Sutter Instruments
Uniblitz

Cooled CCD cameras
Hamamatsu Orca
Photometrics CoolSnap (Roper)
Andor
Diagnostic Instruments (Spot)
Red Shirt
QImaging (Retiga)
Cooke Corporation (Sensicam)

EM-CCD introduction

Deconvolution
Deltavision (Applied Precision)

MetaMorph (Universal Imaging now Molecular Devices)
IP Lab (Scanalytics now BD Biosciences)
OpenLab, Volocity (Improvision)
Nenad Amodaj MicroManage

iMaris (Bitplane)

Molecular Probes (now Invitrogen)
Cytoskeleton, Inc

Clontech
MBL International (CoralHue / Kaede, Dronpa)
Tsien variants

Zeiss objectives

Ocean Optics spectrometer

Microscopy UK

TIRF systems

FRET tutorial

Immunohistochemistry methods
IHC World

Fluorescence spectra
Invitrogen Fluorescence spectra viewer

Electron microscopy
JEOL
Pelco

BIY confocal
National Instruments
Cambridge Technology
NEOS Technologies
Hamamatsu PMTs
Uniblitz / Vincent Associates
Mathworks Matlab
Svoboda lab software

Journal Cell Biology: how to image an article by Alison North

 

Some recent papers by faculty and lecturers
Commons Glutamatergic axons in the raphe nucleus (Eur J Neuroscience)
Kuhn A new voltage sensitive dye (Biophysical J.)
Micheva Effect of sub-physiological temperatures on synaptic function and plasticity (J. Neuroscience)
Misgeld In vivo imaging of injured spinal cord (Nature Medicine)
Oertner Review of "Glow in the Dark" (Nature)
Reese Composition of the post synaptic density (PNAS)
Smith Axonal mechanisms in synaptogenesis in zebrafish brain (J Neuroscience)
Terasaki Permeability changes following membrane repair (J. Gen. Physiol.)
Thompson Transgenic mice with GFP- glia (J Neuroscience)
Zimmerberg Primer: Membrane Biophysics (Current Biology)

Winfried Denk Serial block face scanning electron microscopy to reconstruct three dimensional tissue nanostructure. (PLOS)
Kristin Harris Ultrastructural changes in dendritic spines during long term potentiation (Philos Trans Royal Soc)
Ryohei Yasuda Ras activation in spines observed by 2 photon FLIM (Nature Neurosci)
---- and Neuroscience blog
David Zenisek Coupling between clathrin coated pit invagination, cortactin recruitment, and membrane scission (Cell 121:593 (2005))
Marge Livingstone A cortical region consisting entirely of face-selective cells (Science)

Fritz Zernike Nobel Prize Lecture 1953 "How I discovered phase contrast"
Ernst Ruska Nobel Prize Lecture 1986 "Development of the electron microscope and of electron microscopy"

Keffer Hartline Nobel Prize lecture 1967 Visual receptors and retinal interaction
George Wald Nobel Prize lecture 1967 The molecular basis of visual excitation
Alan Hodgkin Nobel Prize lecture 1963 The ionic basis of nervous conduction
Andrew Huxley Nobel Prize lecture 1963 The quantitative analysis of conduction and excitation in nerve

Giovanni Battista Amici - inventor of oil immersion lens (1840), water immersion lens (1855), amici-bertrand lens for looking at the back focal plane of the objective, cytoplasmic streaming in plants, pollen tubes, among other things

Steve: Remembranes of Stephen W. Kuffler

NB students from previous years (from 1996, not complete)
NB course faculty from previous years (not done yet)

The Professor and the Fish (a story involving Louis Agassiz, a founder of the MBL)

Jacques Loeb
FR Lillie
Charles Whitman